Genotoxicity of the Pesticide Dichlorvos and Herbicide Butachlor on Rana zhenhaiensis Tadpoles

作     者：GENG Baorong LIN Ling ZHANG Qiujin ZHONG Bijin 

作者机构：College of Life SciencesFujian Normal University Higher Education Laboratory of Developmental Biology and NeurobiologyFujian Normal University 

出 版 物：《Asian Herpetological Research》 (亚洲两栖爬行动物研究（英文版）)

年 卷 期：2010年第1卷第2期

页      面：118-122页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：granted by the Natural Science Foundation of Fujian,China(2009J01129) the Education Department Foundation of Fujian,China(2007F5033) 

主　　题：comet assay dichlorvos butachlor DNA damage pesticide herbicide Rana zhenhaiensis 

摘      要：Genotoxicity of dichlorvos and butachlor on erythrocytes of Rana zhenhaiensis tadpoles was investigated by the alkaline single-cell gel electrophoresis assay or comet *** were treated for 24h in the laboratory with different concentrations of the testing agents,2.256,4.512,6.768,9.024,11.280mg/L for dichlorvos and 0.292,0.584,0.876,1.168,1.460mg/L for butachlor,to use the comet Assay to test for the significance of dosage responsiveness to an increase in DNA damage,asmeasured by themean DNA tail length-to-width *** concentrations of 4.512mg/L dichlorvos and 0.876mg/L butachlor resulted in highly significant increases in DNA damage of the *** were linear correlations between themean DNA tail length-to-width ratio and the concentrations of the two test *** results showed that the two commonly used agricultural chemicals caused dose dependent DNA damage of amphibians,and that comet assaymight be a useful tool formeasuring DNA damage of tadpoles exposed in the field.