Identification and preliminary characterization of novel B3-type metallo-β-lactamases

作     者：Manfredi Miraula Conor SBrunton Gerhard Schenk Natasa Mitić 

作者机构：Department of ChemistryNational University of Ireland—MaynoothMaynoothCo.KildareIreland School of Chemistry and Molecular BiosciencesThe University of QueenslandBrisbaneAustralia 

出 版 物：《American Journal of Molecular Biology》 (美国分子生物学期刊（英文）)

年 卷 期：2013年第3卷第4期

页      面：198-203页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：N.M.thanks the Science Foundation Ireland(SFI)for financial support in form of a President of Ireland Young Researcher Award(PIYRA) G.S.acknowledges the award of a Future Fellowship from the Australian Research Council(FT120100694) is grateful to the National Health and Medical Research Council of Australia for funding 

主　　题：Antibiotic Resistance β-Lactam Antibiotics Metallo-β-Lactamases Sequence Homology Novosphingobium Pentaromativorans Simiduia Agarivorans 

摘      要：Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is among the most potent. MBLs are subdivided into three groups (i.e. B1, B2 and B3) with most of the virulence factors belonging to the B1 group. The recent discovery of AIM-1, a B3-type MBL, however, has illustrated the potential health threat of this group of MBLs. Here, we employed a bioinformatics approach to identify and characterize novel B3-type MBLs from Novosphingobium pentaromativorans and Simiduia agarivorans. These enzymes may not yet pose a direct risk to human health, but their structures and function may provide important insight into the design and synthesis of a still elusive universal MBL inhibitor.