Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

作     者：Yimin Zhu Fuying Xu 

作者机构：Department of Surgery College of Medicine and Health Lishui University/Lishui People’s Hospital Lishui Zhejiang 323000 China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2017年第32卷第1期

页      面：44-47页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：gastric carcinoma microRNA apoptosis 

摘      要：Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901. Methods The stable let-7a gene over-expressing SGC-7901 cells,SGC-7901/let-7a cells,were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry. Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells(P=0.002). Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.