CAMSAP3-dependent microtubule dynamics regulates Golgi assembly in epithelial cells

作     者：Jing Wang Honglin Xu Yuqiang Jiang Mikiko Takahashi Masatoshi Takeichi Wenxiang Meng 

作者机构：State Key Laboratory of Molecular Developmental BiologyInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesNo.19A Yuquan RoadBeijing 100049China Faculty of Pharmaceutical SciencesTeikyo Heisei University4-21-2 NakanoNakano-kuTokyo 164-8530Japan RIKEN Center for Developmental Biology2-2-3 Chuo-kuKobe 650-0047Japan 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2017年第44卷第1期

页      面：39-49页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071009[理学-细胞生物学] 

基　　金：supported by the National Natural Science Foundation of China(No.31271426) the National Basic Research Program of China(No.2014CB942802) the Key Research Program of the Chinese Academy of Sciences(No.KJZD-EW-L08)to W.M. Grant-in-Aid for Scientific Research(S)(No.25221104)from the Japan Society for Promotion of Science to M.Takeichi 

主　　题：CG-NAP Nezha/CAMSAP3 Golgi Noncentrosomal micfotubules Stathmin 

摘      要：The Golgi assembly pattern varies among cell types. In fibroblast cells, the Golgi apparatus concentrates around the centrosome that radiates microtubules; whereas in epithelial cells, whose microtubules are mainly noncentrosomal, the Golgi apparatus accumulates around the nucleus independently of centrosome. Little is known about the mechanisms behind such cell wpe-specific Golgi and microtubule organization. Here, we show that the microtubule minus-end binding protein Nezha/CAMSAP3 （calmodulin-regulated spectrin-associated protein 3） plays a role in translocation of Golgi vesicles in epithelial cells. This function of CAMSAP3 is supported by CG-NAP （centrosome and Golgi localized PKN- associated protein） through their binding. Depletion of either one of these proteins similarly induces fragmentation of Golgi membranes. Furthermore, we find that stathmin-dependent microtubule dynamics is graded along the radial axis of ceils with highest activity at the perinuclear region, and inhibition of this gradient disrupts perinuclear distribution of the Golgi apparatus. We propose that the assembly of the Golgi apparatus in epithelial cells is induced by a multi-step process, which includes CAMSAP3-dependent Golgi vesicle clustering and graded microtubule dynamics.