Targeted mutagenesis in rice using CRISPR-Cpf1 system

作     者：Xixun Hu Chun Wang Qing Liu Yaping Fu Kejian Wang 

作者机构：State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural Sciences Hangzhou 310006 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2017年第44卷第1期

页      面：71-73页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the National Natural Science Foundation of China(No.3140101312) the Zhejiang Provincial Natural Science Foundation of China(No.LZ14C130003) the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences 

主　　题：Targeted mutagenesis in rice using CRISPR-Cpf1 system tRNA 

摘      要：Cpf1 is a class 2/type V CRISPR effector that has been recently harnessed for genome editing （Zetsche et al., 2015; Hut et al., 2016; Kim et al., 2016）. Cpff recognizes thymidine-rich sequence as the protospacer-adjacent motif （PAM） at the 5＇ end of target sequences. In addition, Cpfl requires only a single shorter crRNA and cleaves DNA in a staggered fashion with 5＇ overhangs （Zetsche et al., 2015）.