Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

作     者：Alessandro Pistone Daniela Iannazzo Claudia Espro Signorino Galvagno Anna Tampieri Monica Montesi Silvia Panseri Monica Sandri 

作者机构：Department of Engineering University of Messina Messina 98166 Italy Institute of Science and Technology for Ceramics. National Research Council ofltaly Faenza 48018 Italy 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2017年第3卷第1期

页      面：55-59页

核心收录：

学科分类：0810[工学-信息与通信工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0808[工学-电气工程] 07[理学] 0817[工学-化学工程与技术] 08[工学] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

主　　题：Mg doped hydroxyapatite Mesenchymal stem cells Chemotactic/haptotactic factorsBone tissue engineering 

摘      要：Stem cell homing, namely the recruitment of mesenchymal stem cells （MSCs） to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped （Mg-doped） hydroxyapatite （HA） may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors： either the fibronectin fragment III1-C human （FF III1-C）, or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin trans- membrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-（4,5-dimethyl- thiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） test, suggested that stem cells migrate to the MgHA disks in resoonse to the grafted haototaxis stimuli.