A New Detecting Method for the Sequence Difference of CHD1 Gene on Sex Chromosomes Z and W in Domesticated Ducks of China
A New Detecting Method for the Sequence Difference of CHD1 Gene on Sex Chromosomes Z and W in Domesticated Ducks of China作者机构：Key Laboratory of Poultry Heredity & Breeding Jiangsu Institute of Poultry Science Yangzhou 225003 China Jiangsu Tengdayuan Agriculture and Live-stock Co. Ltd. Jiangyan 225538 China
出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)
年 卷 期：2016年第8卷第6期
页 面：327-330页
基 金：Supported by the National Natural Science Foundation of China(31172194) The Science and Technology Support Program of Jiangsu Province(BE2011329) The Variety Innovation Project of Modern Agriculture(CX(11)1030)
主 题：Domesticated duck in China CHDI Sex molecular identification Molecular marker
摘 要：A new method for detecting the sequence differences of duck Chromo-helicase-DNA-Binding 1 （ CHD1 ） genes on sex chromosomes Z and W was established by amplifying DNA fragment length poly morphism, which aimed to solve some technical problems, such as the sex identification with nondamage, the misclassifc rate of artificial sex identification during embryonic period, and the injury caused by anal reversal identification method during neonatal period. The im- proved sex identification method was developed by DNA amplified fragment length polymorphism by using CHD1 gene sequence in bird. The sex identification PCR primers YPF/YPR were used, and the PCR product was cloned, sequenced and blasted; and the effectiveness and accuracy of this method were verified through liv- ing examples. 2% agarose gel electrophoresis was used to distinguish 495 bp CHD1-Z and 351 bp CHD1-W obtained by primer YPF/YPR. The female （ZW） dis- played two visible bands; while male （ZZ） had only one single band. It was proved that this method for detecting the sequence differences of duck CHD1 gene was visual and reliable, and the molecular marker provided was effective and precise in the sex molecular biological identification of domesticated duck in China.
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