Construction of an integrated map and location of a bruchid resistance gene in mung bean

作     者：Lixia Wang Chuanshu Wu Min Zhong Dan Zhao Li Mei Honglin Chen Suhua Wang Chunji Liu Xuzhen Cheng 

作者机构：Institute of Crop Science Chinese Academy of Agricultural Sciences CSIRO Agriculture and Food 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2016年第4卷第5期

页      面：360-366页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the National Natural Science Foundation of China (Nos. 30871565, 31571737) the China Agriculture Research System (No. CARS-09) the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS 

主　　题：Vigna radiata Callosobruchus chinensis Linkage map Resistance Gene mapping 

摘      要：Bruchid beetle(Callosobruchus chinensis) poses a serious threat to the production and storage of mung bean(Vigna radiata). Mapping bruchid resistance(Br) will provide an important basis for cloning the responsible gene(s) and elucidating its functional mechanism, and will also facilitate marker-assisted selection in mung bean breeding. Here, we report the construction of the genetic linkage groups of mung bean and mapping of the Br1 locus using an RIL population derived from a cross between Berken, a bruchid-susceptible line, and ACC41, a bruchid-resistant line. A total of 560 markers were mapped onto 11 linkage groups,with 38.0% of the markers showing distorted segregation. The lengths of the linkage groups ranged from 45.2 to 117.0 c M with a total coverage of 732.9 c M and an average interval of1.3 c M between loci. Br1 was located on LG9 between BM202(0.7 c M) and Vr2-627(1.7 c M).Based on 270 shared SSR markers, most of the linkage groups were assigned to specific chromosomes. These results should further accelerate the genetic study of this crop.