A fluorescent aptasensing strategy for adenosine triphosphate detection using tris(bipyridine)ruthenium(Ⅱ) complex containing six cyclodextrin units

作     者：Xin Nie Xin Ning Ying-Ying Zhao Li-Zhu Yang Fan Zhang Pin-Gang He 

作者机构：School of Chemistry and Molecular Engineering East China Normal University School of Pharmaceutical Sciences Wenzhou Medical University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2017年第28卷第3期

页      面：619-624页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21275054 21405049 31300819) 

主　　题：Fluorescence Aptasensing ATP Metallocyclodextrin Host–guest recognition 

摘      要：A sensitive label-free fluorescent aptasensing strategy for the detection of adenosine triphosphate（ATP）has been developed with a metallocyclodextrin, tris（bipyridine）ruthenium（Ⅱ） complex containing six cyclodextrin units（6CD-Ru）, which exhibited much stronger emission signal compared to the parent compound Ru（bpy）_3Cl_2. Furthermore, the emission spectrum showed that the ATP-aptamer（ss DNA）could increase the fluorescence intensity of 6CD-Ru dramatically, attributed to the interaction between aptamer and cyclodextrin, which could provide protection to the ruthenium core from the quenching of emission by oxygen in the solution. With the addition of ATP, the interaction between aptamer and cyclodextrins on 6CD-Ru was diminished, since the ATP/aptamer complex had the priority to be formed,leading to the corresponding reduction of fluorescence intensity, which could be utilized to detect ATP quantitatively. A linear relationship was displayed between the fluorescence and the logarithm of ATP concentrations in the range from 1 nmol/L to 1μmmol/L with the detection limit of 0.5 nmol/L（S/N = 3）.The proposed fluorescent aptasensing strategy exhibited high sensitivity and specificity, without any labeling or amplification procedures, and it could also be applied for the detection of many other aptamer-specific targets.