Human papillomavirus(HPV) E6/E7 mRNA detection in cervical exfoliated cells:a potential triage for HPV-positive women

作     者：Ye-li YAO Qi-fang TIAN Bei CHENG Yi-fan CHENG Jing YE Wei-guo LU 

作者机构：Department of Gynecologic Oncology Women 's Hospital School of Medicine Zhejiang University Hangzhou 310006 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2017年第18卷第3期

页      面：256-262页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Project supported by the Special Fund for Scientific Research in the Public Interest from the National Health and Family Planning Commission of the People’s Republic of China(No.201402010) the National Key Technology R&D Program of China(No.2016YFC1302900) the Zhejiang Provincial Educational Project(No.Y201328819) the Zhejiang Provincial Medical & Hygienic Science and Technology Project(Nos.2013KYB147 and 2013KYA104) the Zhejiang Provincial Natural Science Foundation(No.LQ14H160007),China 

主　　题：Human papillomavirus (HPV) HPV E6/E7 mRNA High-grade squamous intraepithelial lesion (HSIL) 

摘      要：Cytology triage has been generally recommended for human papillomavirus （HPV）-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou （Pap） test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson＇s Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion （HSIL） cases was higher than that in low-grade squamous intraepithelial lesion （LSIL） or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity （89.52% vs. 86.67%, P=0.671）, specificity （48.96% vs. 48.96%, P=1.000）, positive predictive value （39.00% vs. 38.24%, P=1.000）, and negative predictive value （92.76% vs. 90.97%, P=-0.678） for detecting ≥HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.