Kicking down the ladder： adding cleavable features to genetically encoded photocrosslinkers

作     者：Zhi Lin Xiao Xie Peng R. Chen 

作者机构：Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Synthetic and Functional Biomolecules Center Peking University Beijing 100871 China peking-Tsinghua Center for Life Sciences Beijing 100871 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2017年第60卷第2期

页      面：167-169页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Key Research and Development Program(2016YFA0501500) the National Natural Science Foundation of China(21225206,21432002,21521003) 

主　　题：Chemistry Synthesis (chemical) 

摘      要：Protein-protein interactions(PPIs)play vital roles in biological ***,the discovery of transient and biologically relevant PPIs remain a formidable challenge using conventional strategies such as co-immunoprecipitation(CoIP),yeast two hybrids(Y2H)and Forster resonance energy transfer(FRET)[1].Genetically encoded photocrosslinkers have recently emerged as a powerful approach for probing intracellular PPIs with the capability for in situ studies,low perturbation to cells as well as the wide *** facile strategy also demonstrated an advantage of high spatiotemporal resolution,which offers a robust capture strategy for the discovery of transient and/or weak PPIs with lowered false-positive backgrounds[2].