LC-MS-based Metabolomics in Traditional Chinese Medicines Research: Personal Experiences

作     者：Qian Mao Jin-di Xu Ming Kong Hong Shen He Zhu Shan-shan Zhou Song-lin Li 

作者机构：Department of Pharmaceutical Analysis Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese Medicine Department of Metabolomics Jiangsu Province Academy of Traditional Chinese Medicine 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2017年第9卷第1期

页      面：14-21页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Natural Science Foundation of China(81573596,81503191,81503365,81503246,81274068,81373946 and 81303221) National High Technology Research and Development Plan of China(863 Plan)(2014AA022204) 

主　　题：LC-MS metabolomics traditional Chinese medicines 

摘      要：LC-MS-based metabolomics has being widely used in traditional Chinese medicines （TCMs） research due to the great coverage of mass ranges, high sensitivity to detect metabolites, and no need of sample derivatization. Herein, we reviewed our research on the applications of LC-MS-based metabolomics in TCMs research over the past decade in the following aspects： herbal authentication, determination of herb harvest time, chemical transformation of herbs during post-harvest handlings （sulfurfumigation and drying）, discrimination of raw and processed herbs, chemical transformation of TCMs during preparation, screening endogenous toxic compounds in TCMs, unveiling synergistic mechanisms between small molecules （such as saponins） and polysaccharides in TCMs, revealing synergistic actions of TCMs with chemical drugs, which demonstrated that metabolomics is a superior strategy in TCMs research concerning the holistic perspectives.