Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

作     者：Ali Ahadi Alireza Partoazar Mohammad Hassan Abedi Khorasgani Seyed Vahid Shetab Boushehri 

作者机构：Department of PhysiologySchool of MedicineTehran University of Medical Sciences Department of PharmacologySchool of MedicineTehran University of Medical Sciences Legal Medicine Organization of Iran Iran Department of Medical NanotechnologySchool of MedicineTehran University of Medical Sciences 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2011年第25卷第5期

页      面：362-367页

学科分类：0710[理学-生物学] 12[管理学] 1204[管理学-公共管理] 120402[管理学-社会医学与卫生事业管理(可授管理学、医学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：Merck Co., Inc Legal Medicine Organization of Iran 

主　　题：morphine detection liquid-liquid extraction thin-layer chromatography solid-phase extraction highperformance thin layer chromatography 

摘      要：Liquid-liquid extraction-thin layer chromatography （LLE-TLC） has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction （SPE） is gradually replacing the tra- ditional LLE method. High performance thin layer chromatography （HPTLC） has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method, in extraction and detection of urinary morphine. Fifty-eight urine samples, primarily identified as mor- phine-positive samples by a strip test, ＇were re-screened by LLE-TLC and SPE-HPTLC. The results of LLE-TLC and SPE-HPTLC were then compared with each other. The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples. We further discussed the effect of codeine abuse on TLC analysis of urinary morphine. Regarding the importance of morphine detection in urine, the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories.