Development and Characterization of Elite Doubled Haploid Lines from Two Indica Rice Hybrids

作     者：Rukmini MISHRA Gundimeda Jwala Narashima RAO Ravi Nageswara RAO Pankaj KAUSHAL 

作者机构：Crop Improvement DivisionCentral Rice Research Institute Indian Grassland and Fodder Institute 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2015年第22卷第6期

页      面：290-299页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

基　　金：Authors are thankful to Director, Central Rice Research Institute, Cuttack, India for his support and guidance. Authors are also thankful to Dr. Ashutosh MALL Dr. N.N. JAMBHULKAR (CRRI) for statistical analysis 

主　　题：anther culture doubled haploid indica rice hybrid 

摘      要：Despite significant yield advantage over inbred rice, the adoption rate of hybrid rice in India is very low due to the high seed cost and poor quality of the produce. To alleviate the problem, we initiated a doubled haploid （DH） breeding approach to develop new lines from two elite indica rice hybrids （CRHR5 and CRHR7） through rapid fixation of homozygosity in the recombinants. In vitro culture of the rice anthers resulted in 243 and 186 fertile DH lines of CRHR5 and CRHR7, respectively. Flow cytometric and pollen fertility analyses confirmed the DH ploidy status of the regenerations. Morpho-agronomic evaluation revealed 100% uniformity and stability for all the characters in the DH lines of both hybrids. Nineteen promising DH lines of each hybrid were advanced to A2 generation for yield evaluation. The yield levels of the DH lines ranged from 5 097-6 965 kg/hm^2 for CRHR5 and 5 141-7 235 kg/hm^2 for CRHR7, which were at par or higher than the parental hybrids. Physico-chemical characterization and cooking quality analyses revealed significant and acceptable values for grain length and width, alkali spreading value, amylose content and water uptake ratio of the selected DH lines. Three DH lines, CR5-10, CR5-49, CR5-61 from CRHR5, and four DH lines, CR7-5, CR7-7, CR7-12 and CR7-52 from CRHR7, showed significant grain yield and quality characteristics and have been recommended for multi-location trials for subsequent release as new indica doubled haploid rice varieties.