Antagonistic Activity, Antimicrobial Susceptibility and Potential Virulence Factors of Enterococcus faecalis
Antagonistic Activity, Antimicrobial Susceptibility and Potential Virulence Factors of Enterococcus faecalis作者机构：Center of Agricultural Environmental and Biological Sciences (CCAAB) Federal University of Rec6ncavo da Bahia (UFRB) Cruzdas AImas Bahia 44380000 Brazil
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2015年第9卷第7期
页 面：318-326页
学科分类：090601[农学-基础兽医学] 08[工学] 09[农学] 0906[农学-兽医学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
主 题：Bivalve mollusks probiotics pathogenicity water.
摘 要：Enterococcus faecalis isolates （87） were phenotypically and genotypically identified and subsequently subjected to the antagonism test and antimicrobial susceptibility. The lipolitic, hemolytic and DNAse activities were identified along with the genes gelE, cylL, cylS, ccf, cpd and cob that, encode virulence determinants. Thirty seven percent of isolates inhibited Listeria monocytogenes （CERELA）, Listeria innocuous （CERELA）, Staphylococcus aureus （ATCC25932）, Lactococcus lactis （IL1403）, Micrococcus luteus （ATCC 10240） and Enterococcusfaecalis （ATCC29212）. All strains were sensitive to the ampicillin antibiotic, but 47% were resistant to at least one antimicrobial agent and 6% of isolates presented multidrug resistance. Ninety seven percent of isolates contained the gelE gene, but 77% of these isolates showed gelatinase activity. Presence of cylL and cylS genes was observed in 25% of the isolates, but only 5% presented hemolytic activity. None isolates showed lipase and DNAse activities. Eight percent of isolates contained the ccf gene and 2% showed the presence of the cpd and cob genes. The ability to inhibit pathogenic bacteria, low resistance to antibiotics and absence of virulence factors make some of Enterococcusfaecalis strains characterized in the present study promising for exploitation in other applications such as probiotics in aquaculture.
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