Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding

作     者：Christopher M.Madl Sarah C.Heilshorn 

作者机构：Department of BioengineeringStanford University Department of Materials Science and EngineeringStanford University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2015年第10卷第4期

页      面：514-517页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by the National Institutes of Health(1DP2-OD006477,R01-DK085720,R21-AR062359-01) the National Science Foundation(DMR-0846363) 

主　　题：neurotrophic factors cell-adhesive ligands dorsal root ganglia L1CAM nerve growth factor biomaterials elastin-like proteins 

摘      要：Both matrix biochemistry and neurotrophic factors are known to modulate neurite outgrowth and pathifnding; however, the interplay between these two factors is less studied. While previous work has shown that the biochemical identity of the matrix can alter the outgrowth of neurites in response to neurotrophins, the importance of the concentration of cell-adhesive ligands is unknown. Using engineered elastin-like protein matrices, we recently demonstrated a synergistic effect between matrix-bound cell-adhesive ligand density and soluble nerve growth factor treat-ment on neurite outgrowth from dorsal root ganglia. This synergism was mediated by Schwann cell-neurite contact through L1CAM. Cell-adhesive ligand density was also shown to alter the pathifnding behavior of dorsal root ganglion neurites in response to a gradient of nerve growth factor. While more cell-adhesive matrices promoted neurite outgrowth, less cell-adhesive ma-trices promoted more faithful neurite pathifnding. These studies emphasize the importance of considering both matrix biochemistry and neurotrophic factors when designing biomaterials for peripheral nerve regeneration.