Direct detection of oxygen free radicals produced in the viscera of burned rats using electron paramagnetic resonance spectroscopy

作     者：路卫 陈玉琳 夏照帆 

作者机构：Burn Center Changhai Hospital The Second MilitaryMedical University Shanghai 200433 China 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2002年第5卷第2期

页      面：118-120页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina (No .3 880 791) 

主　　题：Burns Oxygen free radicals Electron paramagnetic r esonance 

摘      要：To detect superoxide anion (O - 2 ) signals in the heart, liver, lung and kidney tissues after burns. Methods: Twenty four male rats were randomized into 4 groups. The rats of experimental groups were immersed in 100℃ water for 15 seconds and 25% third degree burn was created. Thoracotomy or laparotomy was performed at 5, 10, 15, 20 and 30 minutes after burn, and specimens of the heart, lung, liver kidney were obtained for burned rats. The specimens were then preserved in liqu id nitrogen for cryo preservation and detected immediately using EPR. Results: The signals of superoxide O radical appeared in the he art, lung, 1iver and kidney specimens 10 15 minutes after burn. Conclusions: There is a direct evidence of oxygen free radicals (OFRs) injury to viscera of burned rats between 10 15 minutes after burn.