Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction

作     者：WANG Cuicui CAI Wensheng SHAO Xueguang 

作者机构：Research Center for Analytical Sciences College of Chemistry Nankai University Tianjin 300071 P. R. China Key Laboratory of Biosensing and Molecular Recognition Nankai University Tianjin 3000 71 P. R. China State Key Laboratory of Medicinal Chemical Biology Nankai University Tianjin 300071 P. R. China Collaborative Innovation Center of Chemical Science and Engineering Nankai University Tianjin 300071 R R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2016年第32卷第6期

页      面：912-916页

核心收录：

学科分类：07[理学] 

基　　金：the Project of Innovation Team of the Ministry of Education of China 国家自然科学基金 

主　　题：Near-infrared diffuse reflectance spectroscopy Click reaction Cysteine Multivariate calibration Humanserum 

摘      要：Enrichment technique has been proved to be an efficient way to make the near-infrared diffuse reflectancespectroscopy（NIRDRS） suitable for micro analysis. However, low selectivity presented by conventional enrichmentmethods makes the quantitative analysis easy to be affected by the coexisting components. In this study, a specificenrichment method with chemical bonding via thiol-maleimide click reaction was used to achieve the reduction of theinterferences. Taking cysteine as the analyzing target, maleimide-functionalized SiO2 nanoparticles were prepared forthe enrichment of cysteine. Then determination of cysteine in aqueous solution and human serum was studied usingthe partial least squares model built from the NIRDRS spectra of the adsorbate. The results show that the concentra-tion that can be quantitatively detected is as low as 2.0 μg/mL, and the correlation coefficient（R） between thereference and predicted concentration is 0.9871 for the validation samples. The recoveries are in the range of89.5%-113.8% for human serum samples in the concentration range of 0--16.2 μg/mL.