“Three Methods and Three Points” regulates p38 mitogen-activated protein kinase in the dorsal horn of the spinal cord in a rat model of sciatic nerve injury

作     者：Xin Guo Tian-yuan Yu Wong Steven Wen-duan Jia Chi Ma Yan-hong Tao Chao Yang Tao-tao Lv Shuai Wu Meng-qian Lu Jia-li Liu 

作者机构：College of Acupuncture-Moxibustion and TuinaBeijing University of Chinese Medicine College of Taditional Chinese MedicineBeijing University of Chinese Medicine 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第12期

页      面：2018-2024页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81373759 the Natural Science Foundation of Beijing of China,No.7142097 

主　　题：nerve regeneration tuina Three Methods and Three Points phospho p38 mitogen activated protein kinase sciatic nerve injury tumor necrosis factor α interleukin 1β dorsal horn of the spinal cord neural regeneration 

摘      要：Tuina is a traditional Chinese treatment for sensory disturbances caused by peripheral nerve injury and related diseases. Our previous studies showed that tuina regulates relevant regions and indices of the spinal dorsal horn using the Dian, Bo, and Rou method in Yinmen（BL37）, Yanglingquan（GB34）, and Weizhong（BL40）. Treatment prevents muscle atrophy, protects spinal cord neurons, and promotes sciatic nerve repair. The mechanisms of action of tuina for treating peripheral nerve injury remain poorly understood. This study established rat models of sciatic nerve injury using the crushing method. Rats received Chinese tuina in accordance with the principle of ＂Three Methods and Three Points,＂ once daily for 20 days. Tuina intervention reduced paw withdrawal latency and improved wet weight of the gastrocnemius muscle, as well as promoting morphological recovery of sciatic nerve fibers, Schwann cells, and axons. The protein expression levels of phospho-p38 mitogen-activated protein kinase, tumor necrosis factor-α, and interleukin-1β also decreased. These findings indicate that ＂Three Methods and Three Points＂ promoted morphological recovery and improved behavior of rats with peripheral nerve injury.