Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line
Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line作者机构：Institute for Microbiology and Immunology Medical Faculty Ljubljana University of Ljubljana Zalogka 4 Ljubljana 1105 Slovenia Institute for Biomedical Sciences Medical Faculty Maribor University of Maribor Taborska 8 Maribor 2000 Slovenia Research and Control Department MEDEX d o.o. Linhartova 49a Ljubljana 1000 Slovenia Veterinary Stations of Mostar Bigke Polje 106 Mostar 88000 Bosnia and Herzegovina Croatian Institute for Experimental and Translation Oncology Koledinedka 3 Zagreb 10040 Croatia
出 版 物：《Journal of Agricultural Science and Technology(B)》 (农业科学与技术（B）)
年 卷 期：2016年第6卷第1期
页 面：35-47页
学科分类：090603[农学-临床兽医学] 0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 09[农学] 0906[农学-兽医学]
主 题：Propolis royal jelly Forest honey antimicrobial activity human macrophage cell line cell growth promoting activity.
摘 要：Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly （R J） and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis （WSP） Greitl20 or fresh royal jelly （F-RJ） （MiZigoj） and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus （MRSA）, Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage （TLT） cell line. WSP Greitl20, F-RJ （M） and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration （MIC） in mg/mL. The growth promotion activity was measured at optical density （OD） 595 nm. The combination ofWSP Greitl20 with different Forest honeys is better than F-RJ （M） in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit 120 in the Forest honey （1：10） from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greitl20 in the Forest honey from Italy （GI3 = 0.796 ± 0.014 and GI5 = 1.133± 0.022）. Antimicrobial and growth promoting activities are correlated and WSP-dependent.
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