miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus
miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus作者机构：Department of Surgery Flinders University Bedford Park Department of Gastroenterology and Hepatology Flinders University Bedford Park Department of Surgery Erasmus Medical Centre
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第8期
页 面：1036-1044页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by National Health and Medical Research Council Australia
主 题：miRNA Barrett’s esophagus Esophageal adenocarcinoma miR-200 Epithelial to mesenchymal transition Apoptosis Proliferation Epithelium
摘 要：AIM: To investigate miR-200 family expression in Barrett s epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett s epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett s epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P 0.02). The expression of all miR-200 members was lower in Barrett s epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett s epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett s epithelium and esophageal adenocarcinoma (P 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett s epithelium and regulate key neoplastic processes in this epithelium.
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