Effect of transplantation of bone marrow stem cells on myocardial infarction size in a rabbit model

作     者：Li-li Ji Xiao-feng Long Hui Tian Yu-fei Liu 

作者机构：Department of Emergency MedicineDalian Central Hospital Department of Emergency MedicineSecond Affiliated Hospital of Dalian Medical University 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2013年第4卷第4期

页      面：304-310页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by grants from the Scientific Research Plan Project of Liaoning Province(20092250096) Scientific Research Plan Project of Dalian(2010E15SF178) 

主　　题：Bone marrow stem cells Acute myocardial infarction Epicardial transplantation Intravenous transplantation Infarct size Rabbit 

摘      要：BACKGROUND:Intravenous transplantation has been regarded as a most safe method in stem cell *** is evidence showing the homing of bone marrow stem cells(BMSCs) into the injured sites,and thus these cells can be used in the treatment of acute myocardial infarction(Ml).This study aimed to investigate the effect of intravenous and epicardial transplantion of BMSCs on myocardial infarction size in a rabbit ***:A total of 60 New Zealand rabbits were randomly divided into three groups:control group,epicardium group(group Ⅰ) and ear vein group(group Ⅱ).The BMSCs were collected from the tibial plateau in group Ⅰ and group Ⅱ,cultured and *** the three groups,rabbits underwent thoracotomy and ligation of the middle left anterior descending *** elevation of ST segment0.2 mV lasting for 30 minutes on the lead Ⅱ and Ⅲ of electrocardiogram suggested successful introduction of myocardial *** weeks after myocardial infarction,rabbits in group Ⅰ were treated with autogenous BMSCs at the infarct region and those in group Ⅱ received intravenous transplantation of *** the control group,rabbits were treated with PBS following *** weeks after myocardial infarction,the heart was collected from all rabbits and the infarct size was *** heart was cut into sections followed by HE staining and calculation of infarct size with an image ***:In groups Ⅰ and Ⅱ,the infarct size was significantly reduced after transplantation with BMSCs when compared with the control group(P0.05).CONCLUSION:Transplantation of BMSCs has therapeutic effect on ***,epicardial and intravenous transplantation of BMSCs has comparable therapeutic efficacy on myocardial infarction.