Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling

作     者：Qian-nan DIAO Yong-jun SONG Dong-mei SHI Hong-yan QI 

作者机构：Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region Key Laboratory of Protected Horticulture of Ministry of Education and Liaoning Province College of Horticulture Shenyang Agricultural University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2016年第17卷第12期

页      面：916-930页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the China Agriculture Research System(No.CARS-25) the Liaoning Innovative Research Team in University(No.LZ2015025),China 

主　　题：Antioxidant enzymes Chilling tolerance Hydrogen peroxide Nitric oxide Spermidine Tomato 

摘      要：Polyamines（PAs） and nitric oxide（NO） are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine（Put） and spermidine（Spd） on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase（NOS）-like and nitrate reductase（NR） enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor（sodium nitroprusside（SNP）） and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, and ascorbate peroxidase（APX）, and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato＇s response to chilling stress.