Expression of airway mucus-associated proteins in rats with chronic obstructive pulmonary disease with a cold-dryness symptom pattern

作     者：Gao Zhen Halmurat Upur Wang Jing Jing Jing Li Zheng Xu Dan Li Fengsen 

作者机构：Post-doctoral Scientific Research Station of Tradi-tional Chinese Medicine Hospital affiliated to Xinjiang Medical UniversityUrumqi 830000China National Clinical Research Base of Traditional Chinese MedicineTraditional Chinese Medicine Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830000China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2016年第36卷第5期

页      面：671-677页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by China Postdoctoral Science Foundation:Research of Abnormal Savda Syndrome of COPD Based on the Anaysis of Mucin and Aquaporin(No.2014M562532XB) 

主　　题：Pulmonary disease chronic obstructive Cold-dryness syndrome Aquaporins Mucins 

摘      要：OBJECTIVE: To reveal the effects on expression of airway mucus-associated proteins in rats with chronic obstructive pulmonary disease(COPD) and a cold-dryness symptom pattern induced by elastase and ***: The COPD model was established with an elastase dose into the trachea combined with exposure to smoking; the COPD model cold-dryness symptom pattern was further developed by exposure to a cold, dry environment. After 90 days,pathologic lung sections, inflammatory cytokine levels(measured by enzyme linked immunosorbent assay), m RNA and protein expression of mucus-associated proteins and aquaporins(measured by real-time polymerase chain reaction and western blots) were ***: Cytokines interleukin-6(IL-6), interleukin-8(IL-8), and tumor necrosis factor-α(TNF-α) in the COPD and the cold-dryness symptom pattern COPD groups were all significantly higher than in controls(each P 0.01). IL-6 and IL-8 levels were higher in the cold-dryness symptom pattern COPD group than in the COPD group(each P 0.05). The AQP5 m RNA expression in the cold-dryness symptom pattern COPD and COPD groups was lower than in the control group(P 0.01), and that in the cold-dryness symptom pattern COPD group was lower than the COPD group(P 0.05). The expression of MUC5 AC and MUC5 B m RNAs in the cold-dryness symptom pattern COPD group and COPD group was higher than in the control group(each P 0.01), and that in the cold-dryness symptom pattern COPD group was higher than the COPD group(P 0.01, and P 0.05, respectively).The ratio of MUC5 AC m RNA/MUC5 B m RNA was COPD group the cold-dryness symptom pattern COPD group the control group. AQP4 and AQP5 protein expression in the cold-dryness symptom pattern COPD group was lower than that in the COPD group which was lower again than in the control group. MUC5 AC and MUC5 B expression in the cold-dryness symptom pattern COPD group was higher than in the COPD group and higher again than in the control ***