Scleral TGF-β1 and Smad3 expression is altered by TCM Bu Jing Yi Shi Tablets in guinea pigs with form-deprivation myopia

作     者：Ya Mo Yi Wang Bin Cao Jiajia Zhang Guoting Ren Ting Yang 

作者机构：Department of OphthalmologyThe Affiliated Hospital of Chengdu University of TCMChengdu 610072China Department of PathologyThe Affiliated Hospital of Chengdu University of TCMChengdu 610072China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2016年第3卷第2期

页      面：124-132页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the Scientific Research Foundation of Sichuan Provincial Education Department(11ZA065:Scleral TGF-b1 expression in guinea pigs with form-deprivation myopia is altered in response to invigoration spleen and elimination blood stasis) the Department of Public Health Foundation,Sichuan Province(110527:Study on FDM guinea pig scleral fibroblasts TGFb1/Smad3 signaling pathway) the Science and Technology Development Foundation of the Teaching Hospital of Chengdu University of TCM(2012-D-YY-12:Research on FDM:guinea pig retinal function is altered in response to nourishing Xu and removing blood stasis) 

主　　题：TCM Form-deprivation myopia Sclera TGF-b1 Smad3 

摘      要：Objective:To explore whether the traditional Chinese medicine(TCM)Bu Jing Yi Shi Tablets alters the expression of scleral TGF-b1 and Smad3 in guinea pigs with formdeprivation myopia(FDM).Methods:Sixty-five guinea pigs were randomly divided into control,model,low-,medium-,and high-dose treatment *** for the control group,FDM was induced by covering the right eye of each animal with opaque *** treatment groups were gavaged with different suspension concentrations of Bu Jing Yi Shi *** and axial length were performed before and after myopia *** the end of the experiment,all right eyes were extracted,and scleral sections were prepared for staining and TGF-b1 and Smad3 *** thickness and area,the scleral fibroblast quantity,and scleral TGFb1 and Smad3 expressions were ***:The 5 FDM groups had the same initial axial length and diopter,the final diopter and axial length of the model group were significantly increased compared with the control group and treatment groups(P.01).The axial length of each treatment group decreased as the dose decreased compared with the model group(P.01);the total scleral area(P.05 e.01)and the number of scleral fibroblasts(P.01)in the model group were significantly lower than the treatment *** the TGF-b1 and Smad3 integral optical densities in the model group were significantly lower than the control and medium-and high-dose treatment groups(P.01).TGF-b1 and Smad3 mRNAs in the model group were decreased compared with the control group,but increased in expression after treatment.