Informatic analysis reveals Legionella as a source of novel natural products

作     者：Chad W.Johnston Jonathan Plumb Xiang Li Sergio Grinstein Nathan A.Magarvey 

作者机构：The Michael G.DeGroote Institute for Infectious Disease ResearchDepartment of Biochemistry and Biomedical SciencesMcMaster UniversityHamiltonONCanada L8N 3Z5 Department of Chemistry and Chemical BiologyMcMaster UniversityHamiltonONCanada L8N 3Z5 Cell Biology ProgramHospital for Sick ChildrenTorontoOntarioCanada M5G 1X8 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2016年第1卷第2期

页      面：130-136页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：generous gifts from McMaster University and the Canadian Institute of Health Research(CIHR).C.Johnston is funded through a CIHR Doctoral Research Award.N.Magarvey is funded by a CIHR New Investigator Award.We wish to thank Dr.Cyril Guyard for providing L.pneumophila LP02 as well as the GFP-expression plasmid pBH6119 

主　　题：Natural products Legionella Polyketides PRISM 

摘      要：Microbial natural products are a crucial source of bioactive molecules and unique chemical *** their importance,rediscovery of known natural products from established productive microbes has led to declining interest,even while emergent genomic data suggest that the majority of microbial natural products remain to be ***,new sources of microbial natural products must be defined in order to provide chemical scaffolds for the next generation of small molecules for therapeutic,agricultural,and industrial *** this work,we use specialized bioinformatic programs,genetic knockouts,and comparative metabolomics to define the genus Legionella as a new source of novel natural *** show that Legionella *** a diverse collection of biosynthetic gene clusters for the production of polyketide and nonribosomal peptide natural *** confirm this bioinformatic survey,we create targeted mutants of *** and use comparative metabolomics to identify a novel polyketide *** spectroscopic techniques,we show that this polyketide possesses a new chemical scaffold,and firmly demonstrate that this unexplored genus is a source for novel natural products.