The impacts of a fliD mutation on the biofilm formation of Helicobacter pylori

作     者：Panan Ratthawongjirakul Vorraruthai Thongkerd Wanpen Chaicumpa 

作者机构：Department of Transfusion Medicine and Clinical MicrobiologyFaculty of Allied Health SciencesChulalongkorn University Faculty of Allied Health SciencesChulalongkorn University Department of Microbiology and ImmunologyFaculty of Tropical MedicineMahidol University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2016年第6卷第12期

页      面：1008-1014页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by the Thailand Research Fund(MRG5580161) the Research Fund Project(ASHCU57002) the Faculty of Allied Health Sciences at Chulalongkorn University the Graduate School Thesis Grant of Chulalongkorn University 

主　　题：Helicobacter pylori Biofilm Flagella fliD 

摘      要：Objective:To investigate the impact of the fliD gene on the biofilm formation of Helicobacter pylori(***).Methods:*** fliD mutant was constructed using inverse PCR *** mobility of the bacteria and its adhesion ability to human epithelial cells were assessed using a motility assay and a fluorescein isothiocyanate staining adhesion assay,*** formation of biofilm was evaluated using a pellicle assay and a crystal violet staining *** cy to-architecture of the biofilm was documented with scanning electron ***:It was found that there was no significant difference in the levels of bacterial adhesion and the biofilm formation between the wild-type ATCC 43504 and the fliD *** from a poor motility,the fliD mutant had a slightly delayed formation of its biofilm and an incomplete cy to-architecture of its *** bacterial cells residing in the biofilm of the fliD mutant showed a loose accumulation with less apparent crosslinking *** of the mutant cells had truncated ***:This study provides the preliminary evidences that fliD potentially regulates biofilm formation and is required for the motility of *** studies need to be performed in order to develop fliD as a novel target for vaccine or antimicrobial agent in future.