The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection

作     者：Gui-Qiu Zhao Jing Lin Li-Ting Hu Xiao-Ni Yin Qian Wang Qiang Xu Hui Li 

作者机构：Department of Ophthalmologythe Affiliated Hospital of Qingdao University 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2016年第9卷第10期

页      面：1371-1375页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China(No.81170825 No.81470609 No.81500695) Specialized Research Fund for the Doctoral Program of Higher Education(No.20123706110003) the Youth Natural Science Foundation of Shandong Province(No.ZR2013HQ007) the Key Project of Natural Science Foundation of Shandong Province(No.ZR2012HZ001) 

主　　题：Dectin-1/Raf-1 signal pathway Aspergillus fumigatus innate immune human corneal epithelial cells 

摘      要：AIM： To investigate the expression of the v-raf-1murine leukemia viral oncogene homolog 1（Raf-1） and its role in the innate immune response of human corneal epithelial cells（HCECs） infected by Aspergillus ***： HCECs were cultured in *** were randomly divided into 4 groups,including control group,Aspergillus fumigatus group,GW5074（an inhibitor of Raf-1） group and Laminarin [an inhibitor of Dendriti-cell-associated C-type lectin 1（Dectin-1）] *** protein expression level of total Raf-1 and p-Raf-1 was measured by Western *** expression of IL-6 and IL-8 m RNA in each group was detected by real-time polymerase chain ***： In Aspergillus fumigatus group,total Raf-1 protein levels in HCECs remained unchanged at 5,15,30 and 45min after infection,while p-Raf-1 expression was significantly enhanced at 30 min after infection compared with control ***,the expression of p-Raf-1 was apparently declined after treated with GW5074 or Laminarin compared with Aspergillus fumigatus *** expression levels of IL-6,IL-8 m RNA were significantly increased after stimulation with fumigatus compared with control ***-treated with GW5074 significantly inhibited Aspergillus fumigatus-induced upregulation of IL-8 and ***： Aspergillus fumigatus stimulation can elevate the expression of p-Raf-1 in HCECs in ***-1/Raf-1 signal pathway may play a role on regulating the expression of inflammatory cytokines,including IL-6 and IL-8.