Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract作者机构：Department of Pharmacy Faculty of Science Tadulako University Department of Pharmacognosy and Phytochemistry Faculty of Pharmacy Hasanuddin University
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2016年第6卷第11期
页 面：962-966页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by the Ministry of Research Technology and Higher Education Republic of Indonesia through Hibah Bersaing grant(Grant No.2851/UN28/DT/2014)
主 题：Lunasia amara Anticancer HeLa T47D Total alkaloid Lunacrine
摘 要：Objective: To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco(L. amara) and to perform further phytochemical ***: The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide(MTT) method against two human cancer cell lines, cervical cancer cells(He La) and breast cancer cells(T47D). Thin layer chromatography, Dragendorf,acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as ***: The ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on He La and T47 D cancer line cells with the IC50 of 71.15 and79.04 mg/m L, respectively. Total alkaloid in ethyl acetate extract was counted as(10.46 ± 0.28)%(w/w), while lunacrine determined by ultra fast liquid chromatographyphotodiode array detection method was found to be(3.55 ± 0.26)%(w/w).Conclusions: The high total alkaloid and lunacrine concentration on the extract confirm the potential cytotoxic property of ethyl acetate wood extract of L. amara.
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