Nrf2 inhibits epithelial-mesenchymal transition by suppressing snail expression during pulmonary fibrosis

作     者：ZHOU Wen-cheng MO Xiao-ting ZHANG Zhi-hui CUI Wen-hui GAO Jian 

作者机构：School of PharmacyAnhui Medical University The First Affiliated Hospital of Anhui Medical University The Second Hospital of Dalian Medical University 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2016年第30卷第10期

页      面：1036-1036页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：The project supported by National Natural Science Foundation of China(81274172 81473267 30801535 and 81470003) 

主　　题：nuclear factor erythroid 2-related factor2 snail epithelial-mesenchymaltransition pulmonaryfibrosis 

摘      要：OBJECTIVE Epithelial-mesenchymal transition(EMT)is a phenotype conversion that plays a critical role in the development of pulmonary fibrosis(PF).It is known that a transcription factor snail could regulate the progression of *** factor erythroid 2 related factor 2(Nrf2),a key regulator of antioxidant defense system,protects cells and tissues against oxidative ***,it is not known whether Nrf2 regulates snail thereby modulating the development of *** Bleomycin(BLM)was intratracheally injected into both Nrf2-knockout(Nrf2-/-)and wild-type mice to compare the development of *** type II alveolar epithelial cells(AECs)RLE-6TN were treated with a specific Nrf2activator sulforaphane,or transfected with Nrf2 and snail si RNAs to determine their effects on transforming growth factorβ1(TGF-β1)-induced *** BLM-induced EMT and lung fibrosis were more severe in Nrf2-/-mice compared to wild-type *** vitro,sulforaphane treatment attenuated TGF-β1-induced EMT,accompanied by the down-regulation of ***,silencing Nrf2 by si RNA enhanced TGF-β1-induced EMT along with the expression of ***,silencing snail by si RNA reduced TGF-β1-induced EMT even in the presence of sulforaphane in RLE-6TN *** These findings suggested that Nrf2 may attenuate EMT and fibrosis process through regulating the expression of snail in PF.