Immune response in Balb/c mice induced by recombinant spike protein of severe acute respiratory syndrome coronavirus

作     者：QINLi WUShao-ting WANGXi-ming YUANShi-shan HUANGDa-na LEIMing-jun PANHui-rong LINQi-ping GAOShi-tong ZHANGRen-li QUShen 

作者机构：DepartmentofBiochemistryandMolecularBiologyTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China ShenzhenCenterforDiseaseControlandPreventionShenzhen518020China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2005年第118卷第10期

页      面：857-860页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：ThisresearchwassupportedbyagrantfromtheScienceFoundation forSARSofShenzhencity[No.(2003)74] 

主　　题：severe acute respiratory syndrome coronavirus spike protein IgG isotype T lymphocyte subset cytokine 

摘      要：Severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly aggressive pathogen that caused SARS in 2003 and 2004.^(1-4) Spike protein (S) on the surface of virus particles mediates the attachment of the virus to cell surface receptors and induces the fusion of viral and cellular membranes. According to the epitope analysis and structure of S protein, we used two fragments of S protein S1 (108-488aa) and S2 (723-938aa) expressed in Escherichia coli and immunized Balb/c mice to investigate the immune response to the recombinant proteins in mice.~5