Identification and expression analysis of group Ⅲ WRKY transcription factors in cotton

作     者：DOU Ling-ling GUO Ya-ning Ondati Evans PANG Chao-you WEI Heng-ling SONG Mei-zhen FAN Shu-li YU Shu-xun 

作者机构：College of AgronomyNorthwest A & F University State Key Laboratory of Cotton Biology/Institute of Cotton ResearchChinese Academy of Agricultural Siences 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第11期

页      面：2469-2480页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：the National High-Tech R&D Program of China(2013AA102601)for the financial support provided to this project 

主　　题：Gossypium WRKY phylogenetic analysis expression analysis development abiotic stress 

摘      要：The WRKY proteins constitute a large family of transcription factors in plants containing highly conserved WRKYGQK sequences and zinc-finger-like motifs. To comprehensively study WRKY III genes in cotton, we analyzed the genome sequences of Gossypium hirsutum, G. raimondii and G. arboreum. According to the three genome sequences, 18 group III Gh WRKY genes were identified in G. hirsutum, 12 both in G. raimondii and G. arboreum. Phylogenetic and motif analysis showed that proteins with high similarities could be clustered together and had the same motif components. The ratios of non-synonymous（Ka） to synonymous（Ks） of the Gh WRKY to Gr WRKY or Ga WRKY were lower than 1, which indicated that group III WRKY genes in Gossypium species are under purifying selection. Expression analysis revealed that group III Gh WRKY genes expressed during fiber development and leaf senescence, and most of them could be induced by salicylic acid（SA）, jasmonic acid（JA）, ethylene, abscisic acid（ABA）, mannitol, and Na Cl both in roots and cotyledons. Our study gives a briefly introduction on cotton group III WRKY genes and implicates their potential function in cotton fiber development, leaf senescence and abiotic stresses.