Isoliquiritigenin suppresses microglial activation and neuroinflammation in primary microglia

作     者：FU Yan YANG Pin ZHAO Yang XU Ying 

作者机构：Department of PhysiologyShanghai University of Traditional Chinese Medicine 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2016年第30卷第10期

页      面：1024-1024页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：The project supported by National Natural Science Foundation of China(81274119) 

主　　题：isoliquiritigenin primary microglia inflammatory factors neuroinflammation 

摘      要：OBJECTIVE Microglial activation contributes to neuroinflammation and neuronal damages in neurodegenerative disorders including Alzheimer and Parkinson *** has been suggested that neurodegenerative disorders may be improved if neuroinflammation can be ***(ISL)isolated from Glycyrrhiza glabra possess potent anti-inflammatory capability,we thus investigate the inhibitory effects of ISL on LPS-induced microglial activation and neuroinflammation and the roles of neuroprotection on neurodegenerative ***,we would explore the mechanism of itsassociated inflammatory signaling *** By cultivating,isolating and purifying,we got the primary microglia of the *** form of those cells was observed under an optical microscope,the purity was identified by the CD11b immunefluorescence ***,the effects of ISL on microglial viability were explored by the MTT *** microglial cells were pretreated by ISL(5μmol·L-1)and then stimulated by LPS(100 ng·mL-1),the production of NO was detected by Griess reagent,the change of microglial morphology was observed by immunefluorescence *** production of IL-1βand TNF-αin culture medium were tested by ELISA,and the m RNA expression of pro-inflammatory factors,including IL-1β,TNF-α,i NOS and COX-2 was detected by real time *** protein expression of i NOS and COX-2was detected by Western ***(1)After purifying,microglial showed the typical morphological *** cell body was small,protruding outstretched in the branch rod,*** LPS stimulation,the shape was changed to amoeba-like,and the protrusions retracted,cell *** the CD11b immunefluorescence staining,the purity of the microglial was over 98%,and showed powerful phagocytic activity.(2)The results show that ISL(0.16-20μmol·L-1)concentration has no effect on primary microglia′s *** at the concentration of 5μmol·L-1ISL has the significantly inhibitory effect on NO