In vitro germination and shoot proliferation of the threatened species Cinchona officinalis L(Rubiaceae).

作     者：Rosa Armijos-Gonzalez Cesar Perez-Ruiz 

作者机构：Departamento de Ciencias NaturalesUniversidad Tecnica Particular de LojaCP 1101 608 LojaEcuador Departamento de Biotecnologia y Biologia VegetalE.T.S.Ingenieros AgronomosUniversidad Politecnica de MadridCP 728040 MadridSpain 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2016年第27卷第6期

页      面：1229-1236页

核心收录：

学科分类：09[农学] 

基　　金：the project‘‘PROY_IECOLOGIA_0036’’financed by the third internal call for projects of the Universidad Tcnica Particular de Loja(UTPL) 

主　　题：Callus Cinchona officinalis Germination Phenols Rubiaceae Shoot 

摘      要：Cinchona officinalis （Rubiaceae） is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nine- teenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently, natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C. officinalis seeds were compared with a control species, C. pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L^-1 6-benzyl-aminopurine and 3.0 mg L^-1 indole- 3-butyfic acid.