Suppression of Jasmonic Acid-Mediated Defense by Viral-Inducible MicroRNA319 Facilitates Virus Infection in Rice

作     者：Chao Zhang Zuomei Ding Kangcheng Wu Liang Yang Yang Li Zhen Yang Shan Shi Xiaojuan Liu Shanshan Zhao Zhirui Yang Yu Wang Luping Zheng Juan Wei Zhenguo Du Aihong Zhang Hongqin Miao Yi Li Zujian Wu Jianguo Wu 

作者机构：Fujian Province Key Laboratory of Plant Virology Institute of Plant Virology Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China The State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China / Plant Protection Institute Hebei Academy of Agriculture and Forestry Sciences Baoding 071000 China These authors contributed equally to this article. 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2016年第9卷第9期

页      面：1302-1314页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：福建省自然科学基金 supported by grants to J.W., Z.W., and L.Y. from the National Basic Research Program 973 国家自然科学基金 the Outstanding Young Scholars of Fujian Province Outstanding Young Scientific Research Plan in Higher Education of Fujian Province 

主　　题：miR319 rice ragged stunt virus RRSV TCP21 viral infection jasmonic acid 

摘      要：MicroRNAs （miRNAs） are pivotal modulators of plant development and host-virus interactions. However, the roles and action modes of specific miRNAs involved in viral infection and host susceptibility remain largely unclear. In this study, we show that Rice ragged stunt virus （RRSV） infection caused increased accumulation of miR319 but decreased expression of miR319-regulated TCP （TEOSINTE BRANCHED/ CYCLOIDEA/PCF） genes, especially TCP21, in rice plants. Transgenic rice plants overexpressing miP,319 or downregulating TCP21 exhibited disease-like phenotypes and showed significantly higher susceptibility to RRSV in comparison with the wild-type plants. In contrast, only mild disease symptoms were observed in RRSV-infected lines overexpressing TCP21 and especially in the transgenic plants overexpressing miR319- resistant TCP21. Both RRSV infection and overexpression of miR319 caused the decreased endogenous jasmonic acid （JA） levels along with downregulated expression of JA biosynthesis and signaling-related genes in rice. However, treatment of rice plants with methyl jasmonate alleviated disease symptoms caused by RRSV and reduced virus accumulation. Taken together, our results suggest that the induction of miR319 by RRSV infection in rice suppresses JA-mediated defense to facilitate virus infection and symp- tom development.