Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter

作     者：CHE NanYing,YANG Yang,LI YanDong,WANG LiLi,HUANG Ping,GAO Yin & An ChengCai The National Laboratory of Protein Engineering and Plant Genetic Engineering,School of Life Sciences,Peking University,Beijing 100871,China 

作者机构：The National Laboratory of Protein Engineering and Plant Genetic Engineering School of Life Sciences Peking University Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2009年第52卷第9期

页      面：854-863页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Supported by the Grants from Toyota Motor Corporation of Japan and the National Special Project of Transgenic Organisms (Grant No. 2008ZX08010-001) of the Chinese Government 

主　　题：OLEOSIN LEC2 gene expression seed-specific gene RY element B3 domain transcription factor 

摘      要：As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5 -deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.