COLIPHAGE M13 CLONING SYSTEM AND ddXTP CHAIN-TERMINATION METHOD FOR DNA SEQUENCING

作     者：齐义鹏 QI YIPENG(Department of Virology, Wuhan University)

作者机构：Department of Virology Wuhan University 

出 版 物：《中国科学B辑(英文版)》 (Science in China,Ser.B)

年 卷 期：1986年第29卷第6期

页      面：609-617页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：sequenced recombination cloning DNA Sanger insert transfection yeast recipient restr 

摘      要： Two DNA fragments of cytochrome b gene in yeast mitochondral DNA were sequenced by Messing’s M13 cloning system and Sanger’s ddXTP chain-termination method. M13mp8 and M13mp9 serve as vectors for insert fragment. The recipient strain is E. coli JM103 for transfection. When the ratio of insert/vector was 3:1, high frequencies of recombination and positive recombination were obtained. The two fragments, which have 575 bp and 709 bp, were sequenced. To read more bases, the ratio of ddXTP/dXTP must be reduced. On one X-ray film of 80×17cm 394 bp were read.