GENETIC ANALYSIS OF THE NITROGEN FIXATION SYSTEM IN KLEBSIELLA PNEUMONIAE

作     者：薛中天 金润之 余怡怡 陈惠珠 李武军 沈美静 江群益 沈善炯 HSUEH CHUNG-TIEN CHIN JUN-CHIH YU YI-YI CHEN HUI-CHU LI WU-CHUN SHEN MEI-CHING CHIANG CHUN-YI SHEN SAN-CHIUN (Laboratory of Nitrogen Fixation, Shanghai Institute of Plant Physiology)

作者机构：Laboratory of Nitrogen Fixation Shanghai Institute of Plant Physiology Shanghai Institute of Plant Physiology 

出 版 物：《中国科学A辑(英文版)》 (Science in China,Ser.A)

年 卷 期：1977年第6期

页      面：807-817页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：plasmid Klebsiella transduction histidine assimilation complement nearby Phage maximal glutamate 

摘      要： Fine struture mapping of nif mutations of Klebsiella pneumoniae was accomplished by means of Pl-transductional crosses and the plasmid R144 drd mediated conjugations. The physical distance between nif mutations based on the percentage of co-transduction with hisD of the nif mutations was estimated. The maximal distance between two mutations was calculated about 3 Kb, and the average distance between different nif mutations was about 1 to 2 Kb. So no silent region was shown within the nif cluster nearby the histidine *** hisD-unlinked nif mutants were isolated and investigated genetically and biochemically. They all differed from the glutamineless mutants, one of these mutants was tentatively assigned as a sort of N-assimilation mutant with little activity of glutamate synthetase. It differed from the known N-assimilation mutants in its absence of nitrogenase activity. Since the wild type hisD-linked nif genes carried by the plasmid RP4 failed to complement the defects of the hisD-unlinked n