Rhubarb extract partially improves mucosal integrity in chemotherapy-induced intestinal mucositis

作     者：Juliana E Bajic Georgina L Eden Lorrinne S Lampton Ker Y Cheah Kerry A Lymn Jinxin V Pei Andrea J Yool Gordon S Howarth 

作者机构：Discipline of PhysiologyFaculty of Health SciencesSchool of MedicineThe University of Adelaide Adelaide 5005Australia School of Animal and Veterinary SciencesThe University of AdelaideRoseworthy Campus 5371Australia Gastroenterology DepartmentWomen's and Children's HospitalNorth Adelaide 5006 Australia 2nd Gastroenterology Department Women's and Children's HospitalNorth Adelaide 5006 Australia 2nd School of Animal and Veterinary SciencesThe University of Adelaide Roseworthy Campus 5371Australia 3rd Gastroenterology DepartmentWomen's and Children's HospitalNorth Adelaide 5006 Australia 4th Women's and Children's Health Research InstituteWomen's and Children's HospitalNorth Adelaide 5006Australia 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2016年第22卷第37期

页      面：8322-8333页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Fluorouracil Inflammation Mucositis Rats Rheum 

摘      要：AIM To investigate the effects of orally gavaged aqueous rhubarb extract(RE) on 5-fluorouracil(5-FU)-induced intestinal mucositis in rats. METHODS Female Dark Agouti rats(n = 8/group) were gavaged daily(1 mL) with water, high-dose RE(HDR; 200 mg/kg) or low-dose RE(LDR; 20mg/kg) for eight days. Intestinal mucositis was induced(day 5) with 5-FU(150 mg/kg) via intraperitoneal injection. Intestinal tissue samples were collected for myeloperoxidase(MPO) activity and histological examination. Xenopus oocytes expressing aquaporin 4 water channels were prepared to examine the effect of aqueous RE on cell volume, indicating a potential mechanism responsible for modulating net fluid absorption and secretion in the gastrointestinal tract. Statistical significance was assumed at P 0.05 by one-way ANOVA. RESULTS B o d y w e i g h t w a s s i g n i f i c a n t l y r e d u c e d i n r a t s administered 5-FU compared to healthy controls(P 0.01). Rats administered 5-FU significantly increased intestinal MPO levels(≥ 307%; P 0.001), compared to healthy controls. However, LDR attenuated this effect in 5-FU treated rats, significantly decreasing ileal MPO activity(by 45%; P 0.05), as compared to 5-FU controls. 5-FU significantly reduced intestinal mucosal thickness(by ≥ 29% P 0.001) as compared to healthy controls. LDR significantly increased ileal mucosal thickness in 5-FU treated rats(19%; P 0.05) relative to 5-FU controls. In xenopus oocytes expressing AQP4 water channels, RE selectively blocked water influx into the cell, induced by a decrease in external osmotic pressure. As water efflux was unaltered by the presence of extracellular RE, the directional flow of water across the epithelial barrier, in the presence of extracellular RE, indicated that RE may alleviate water loss across the epithelial barrier and promote intestinal health in chemotherapy-induced intestinal *** In summary, low dose RE improves selected parameters of mucosal integrity and r