In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera ***
In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr,fruit作者机构：Laboratory of Natural Products ResearchDepartment of PharmacyJahangirnagar University
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2014年第4卷第6期
页 面：473-479页
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by Laboratory of Natural Products Research.Jahangirnagar University Dhaka Bangladesh
主 题：Diabetes mellitus Hypoglycemic Citrus macroptera α-Amylase OGTT Glibenclamide
摘 要：Objective:To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr,fruit inα-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents,α-amylase inhibitory activity,and hypoglycemic effect in normal rats and glucose induced hyperglycemic ***:Presence of saponin,steroid and terpenoid were identified in the *** results showed that fruit extract had moderateα-amylase inhibitory activity[IC_(50)value=(3.638±0.190)mg/mL]as compared to *** at 500 mg/kg and 1000 mg/kg doses fruit extract significantly(P0.05 and P0.01 respectively)reduced fasting blood glucose level in normal rats as compared to glibenclamide(5 mg/kg).In oral glucose tolerance test,500 mg/kg dose significantly reduced blood glucose level(P0.05)at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h(P0.05 and P0.01 respectively)whereas glibenclamide(5 mg/kg)significantly reduced glucose level at every hour after *** time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance ***:These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
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