HAEMOPOIETIC PROGENITOR CELLS MEASURED BY LIMITING DILUTION IN MICROWELL CULTURE

作     者：万景华,ALEXANDER A.MORLEY 

作者机构：Institute of Haematology Chinese Academy of Medical Sciences Tianjin Department of Haematology Flinders Medial Centre Adelaide. Australia 

出 版 物：《Science China Chemistry》 (中国科学:化学(英文版))

年 卷 期：1985年第28卷第10期

页      面：1070-1078页

主　　题：well HPC HAEMOPOIETIC PROGENITOR CELLS MEASURED BY LIMITING DILUTION IN MICROWELL CULTURE 

摘      要：Microwell culture assays, termed the negative well technique and the positive well technique have been used to measure the concentration of murine haemopoietic progenitor cells （HPC） by limiting dilution. Using morphologic examination and3H-TdR uptake, the number of HPC of Balb/c mice per 10～5 bone marrow cells was 33 and 31 respectively. The linear relation between the number of harvested cells and input ceils seen on the 4th and 7th days of culture reflected the growth of HPC in microwells