In vitro Effect of Dithiocarbamate Pesticides and of CaNa_2EDTA on Human Serum Dopamine-β-hydroxylase

作     者：PAOLA DE PARIS AND STEFANO CAROLDI(Istituto di Medicina del Lavoro, Universita’ di Padova, Padova, Italy)(Send correspondence to: Dr. Stefano Caroldi. Istituto di Medicina del Lavoro, Via Facciolati 71,I-35127, Padova, Italy. Tel: 3949-8216652, Fax: 3949- PAOLA DE PARIS AND STEFANO CAROLDI(Istituto di Medicina del Lavoro, Universita’ di Padova, Padova, Italy)(Send correspondence to: Dr. Stefano Caroldi. Istituto di Medicina del Lavoro, Via Facciolati 71,I-35127, Padova, Italy. Tel: 3949-8216652, Fax: 3949-

作者机构：Istituto di Medicina del Lavoro Università di Padova Italy. 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：1995年第8卷第2期

页      面：114-121页

核心收录：

学科分类：100405[医学-卫生毒理学] 090403[农学-农药学(可授农学、理学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 09[农学] 0904[农学-植物保护] 10[医学] 

主　　题：EDTA 络合试剂 hydroxylase 

摘      要：Serum dopamine-β-hydroxylase (DBH) inhibition has been reported in lead workers treated with CaNa2EDTA and in alcoholic patients repeatedly treated with the alcohol aversive drug Disulfiram. The mechanism of inhibition involves Cu-chelation at the active site of DBH. The effect of CaNa2EDTA and Disulfiram on serum DBH has been compared to the effect of dithiocarbamate pesticides in vitro for the possible use of serum DBH determination for the biological monitoring of workers exposed to these *** dithiocarbamates inhibit human serum DBH at micromoIar concentrations (range of I50, 0.027-1.6 μmol/L). The inhibitory potency increased from methyl- and dimethyl dithiocarbamates to diethyl dithiocarbamates up to the most potent ethylene bisdithiocarbamates. The I50 of *** was 3.8 mol/L, higher than those of dithiocarbamates. Copper addition to the test system rcactivated at stoichiometric concentrations dithiocarbamaie-inhibited DBH indicating that both base line values and percent of inhibition can be calculated in a single blood sample. Results suggest that serum DBH determination couId be useful in case of acute poisoning involving high doses of dithiocarbamate pesticides