Does progesterone show neuroprotective effects on traumatic brain injury through increasing phosphorylation of Akt in the hippocampus?
Does progesterone show neuroprotective effects on traumatic brain injury through increasing phosphorylation of Akt in the hippocampus?作者机构:Department of NeurosurgeryUniversity of Texas Health Science CenterSan AntonioTXUSA Research Imaging InstituteUniversity of Texas Health Science CenterSan AntonioTXUSA Department of Cellular and Structural BiologyUniversity of Texas Health Science CenterSan AntonioTXUSA Department of NeurologyUniversity of Texas Health Science CenterSan AntonioTXUSA
出 版 物:《Neural Regeneration Research》 (中国神经再生研究(英文版))
年 卷 期:2014年第9卷第21期
页 面:1891-1896页
核心收录:
学科分类:1002[医学-临床医学] 100210[医学-外科学(含:普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金:NCATS NIH HHS [UL1 TR001120 KL2 TR001118] Funding Source: Medline
主 题:nerve regeneration Akt traumatic brain injury progesterone apoptosis neuroprotec-tion brain injury western blotting controlled cortical impact neural regeneration
摘 要:There are currently no federally approved neuroprotective agents to treat traumatic brain injury. Progesterone, a hydrophobic steroid hormone, has been shown in recent studies to exhibit neu-roprotective effects in controlled cortical impact rat models. Akt is a protein kinase known to play a role in cell signaling pathways that reduce edema, inlfammation, apoptosis, and promote cell growth in the brain. This study aims to determine if progesterone modulates the phosphor-ylation of Aktvia its threonine 308 phosphorylation site. Phosphorylation at the threonine 308 site is one of several sites responsible for activating Akt and enabling the protein kinase to carry out its neuroprotective effects. To assess the effects of progesterone on Akt phosphorylation, C57BL/6 mice were treated with progesterone (8 mg/kg) at 1 (intraperitonally), 6, 24, and 48 hours (subcutaneously) post closed-skull traumatic brain injury. The hippocampus was harvest-ed at 72 hours post injury and prepared for western blot analysis. Traumatic brain injury caused a signiifcant decrease in Akt phosphorylation compared to sham operation. However, mice treat-ed with progesterone following traumatic brain injury had an increase in phosphorylation of Akt compared to traumatic brain injury vehicle. Our ifndings suggest that progesterone is a viable treatment option for activating neuroprotective pathways after traumatic brain injury.