BIOLOGICAL FUNCTION OF MODIFIED NUCLEOTIDES IN tRNA MOLECULES——SYNTHESIS AND BIOLOGICAL ACTIVITY OF THE ANALOGUES OF YEAST ALANYL tRNA WITH I34 REPLACED BY A34 OR G34

作     者：裘慕绥 金由辛 李文琴 包俊茹 龚佩娟 吴仁龙 郑可沁 王德宝 QIU MUSUI;JINYOUXIN;LI WENQIN;BAO JUNRU;GONG PEIJUAN;WU RENLONG;ZHENG KEQIN;WANG DEBAO Shanghai Institute of Biochemistry, Academia Sinica

作者机构：Shanghai Institute of Biochemistry Academia Sinica 

出 版 物：《Science in China,Ser.B》 (中国科学B辑(英文版))

年 卷 期：1988年第31卷第6期

页      面：695-701页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China 

主　　题：tRNA BIOLOGICAL FUNCTION OF MODIFIED NUCLEOTIDES IN tRNA MOLECULES OR G 

摘      要：Analogues of yeast alanyl tRNA with I34 replaced by A34 or G34 were synthesized. Synthetic analoguesof yeast alanyl tRNT occupy the same position as the natural yeast alanyl tRNA on polyacrylamide gelelectrophoresis, and their purity is about 95% after electrophoresis on a 10% or 20% polyacrylamide *** two terminal and nearest neighbour nucleotides of the analogues are all correct. The accepting acti-vity of the synthetic analogues is similar to that of the reconstituted natural yeast alanyl tRNA. The in-corporation activity of alanine into proteins of the synthetic analogues is about 30% of that of the naturalof reconstituted natural yeast alanyl tRNA when I34 is replaced by A, and is 90% when I34 is replaced *** reason of the variation in biological function of the analogues of yeast alanyl tRNA after I34 re-placed by A or G was discussed.