Gene Transfer into Mouse Fibroblast and Human Leukemic Cells by Liposomes

作     者：谢久永 彭仁玲 褚嘉佑 吴旻 沈子威 

作者机构：Beijing 100021 Beijing 100086 CAMS Department of Biological Science and Biotechnology National Loboratory of Molecular Oncology Institute PRC Tsinghua University 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：1993年第3卷第2期

页      面：105-108页

核心收录：

基　　金：Project supported by State Commission of Science and Technology and partly by World Laboratories 

主　　题：gene transfer gene carrier liposome 

摘      要：Plasmids,***2-neo and pUCEJ-ras,are entrapped at an efficiency of 63.8% in DOPC(Dioleoyl Phosphatidylcholine)liposomes with intactness by dehydration-rehydration method,andthen transferred into mouse fibroblast cell NIH/3T3(pSV2-neo+pUCEJ-ras)and human leukemic cell CEM(pSV2-neo only).Integration of foreign genes into recipient calls and their expression is demonstrated byG418 selection,DNA hybridization and nude mice inoculation,suggesting the possibility of applying liposomesto target or in vivo gene transfer.