A SPIN LABELING STUDY OF CALF THYMUS DNA
A SPIN LABELING STUDY OF CALF THYMUS DNA作者机构：Shanghai Institute of Biochemistry Academia Sinica
出 版 物：《Science China Chemistry》 (中国科学:化学(英文版))
年 卷 期：1987年第30卷第9期
页 面：946-959页
核心收录：
主 题：A SPIN LABELING STUDY OF CALF THYMUS DNA ESR
摘 要：2,2,6,6-Tetramethyl-4-(bis-ethyteneimino) triazinamino-piperidine-1-oxyl was used as the spin label. In ESR spectra of the product obtained (SL-DNA), the strongly immobilized peak greatly prevailed over the weakly immobilized component and a significant difference existed between their microwave saturation characteristics. The spin labeling reaction was investigated in some details. With the aid of beat denaturation (100℃, 15 min) of SL-DNA in conjunction with acid degradation (pH 1.5, 37℃, 23h), the quantity of labels bound to DNA was determined. Namely, the number of labels bound to 1000 nucleotides was found to be 4.0—12.6. The structural transition temperature of SL-DNA attained from variable temperature measurements was about 63℃ and a rise in this temperature was observed with increasing salt concentration.
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