Fibrinogen and P-selectin expression in atherosclerosis model of Sprague Dawley rat

作     者：ZHOU Bi-rong PAN Ying ZHAI Zhi-min 

作者机构：Department of Hematology Second Affiliated Hospital of Anhui Medical University Hefei Anhui 230601 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2011年第124卷第22期

页      面：3768-3772页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：国家教育部高等学校博士学科点专项科研基金 the Intercollegiate Key Project of Nature Science of Anhui Province the Youth Research Program of Anhui Provincial Health Department 

主　　题：fibrinogen P-selectin atherosclerosis 

摘      要：Background Platelet P-selectin plays an important role in inflammation and contributes to thrombosis and hemostasis. Fibrinogen may take part in inflammation, thrombosis, and hemostasis via enhancement of platelet P-selectin expression. This study aimed to discover the correlation between them in atherosclerosis model of Sprague Dawley （SD） rat. Methods Diet-induced atherosclerosis SD rats were adopted as experimental models. The blood from the common abdominal aorta of the rats was obtained to measure the biochemical characteristics and for the check of flow cytometry. Then the aortas were separated carefully, taken out, put into 10% （w/v） neutral formalin for later use. Then fibrinogen and P-selectin expression were detected by flow cytometry and immunohistochemistry. Results SD rats were induced to atherosclerosis model by high fat diet and vitamin D2 injected. It was discovered that the binding of fibrinogen and the expression of P-selectin on the platelet increase in atherosclerosis model （Group H） than in that in the control group （Group Z）, there were closely interrelated. High levels of fibrinogen and P-selectin express on the artery of atherosclerosis rat model. Conclusions Fibrinogen and P-selectin are concerned with atherosclerosis. Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis.