Assessment of Patulin Content in Apple Puree and Apple and Fruit Puree by High Performance Liquid Chromatography

作     者：Monica Catana Luminita Catana Enuta Iorga Mioara Negoita Valentin Ionescu Nastasia Belc Dragos Efstatiade Hassan Y. Aboul-Enein 

作者机构：National R & D Institute for Food Bioresources-IBA 6 Dinu Vanita Street Bucharest 2021102 Romania Pro Analysis Systems 29 Sfanta Vineri Street Bucharest 030203 Romania Department of Pharmaceutical and Medicinal Chemistry The Pharmaceutical and Drug Industries Research Division NationalResearch Centre Dokki Giza 12622 Egypt 

出 版 物：《Journal of Environmental Science and Engineering(A)》 (环境科学与工程（A）)

年 卷 期：2016年第5卷第7期

页      面：371-381页

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 083203[工学-农产品加工及贮藏工程] 

主　　题：Patulin HPLC-DAD apple puree method performance parameters. 

摘      要：An analytical method was developed and validated for determination of patulin in apple puree by HPLC. Extraction and clean-up ofpatulin from clear extract are achieved on AFFINIMIP SPEPATULIN cartridges. Patulin is then separated on a Hypersil GOLD column 150 mm × 4 mm, 5 μtmand detected at 276 nm. The recovery in the range of 5 μg/kg-80 μg/kg was 81.47%. The limit of detection （LOD） was 1.36 μg/kg, and the limit of quantification （LOQ） was 4.55 μg/kg. The patulin content of the commercial samples of apple puree and samples of apple and fruit puree forinfants and young children as well as the samples of apple puree prepared from two apple varieties intended for processing （Jonathan, Florina） and obtained from conventional and uncertified organic cultures has been evaluated in this paper. The 44.83% patulin concentration of the analyzed samples were under the maximum level of the European Commission Regulation （EC） 1881/2006, in 46.55% of the analyzed samples patulin was not detected and in 8.62% of samples patulin concentration was lower than LOQ （European Comission, 2006a） Patulin was not detected in samples of apple puree intended for infants and young children consumption.