Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene

作     者：Xiu-Feng LI Chong HAN Cai-Rong ZHONG Jun-Qiu XU Jian-Rong HUANG 

作者机构：School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China Management Bureau of Dongzhaigang Mangrove Natural Reserve Haikou 571129 China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2016年第37卷第5期

页      面：307-312页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：funded by the GEF China Wetlands System Project,Science and Technology Foundation of Macao(045/2010/A) Special Fund for Marine-Scientific Research in the Public Interest(201305021) 

主　　题：Sphaeroma terebrans DNA barcode COl gene Molecular identification 

摘      要：Sphaeroma terebrans, a wood-boring isopoda, is distributed worldwide in tropical and subtropical mangroves. The taxonomy of S. terebrans is usually based on morphological characteristics, with its molecular identification still poorly understood. The number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod are considered as the major morphological characteristics in S. terebrans, which can cause difficulty in regards to accurate identification. In this study, we identified S. terebrans via molecular and morphological data. Furthermore, the validity of the mitochondrial cytochrome c oxidase subunit I （COl） gene as a DNA barcode for the identification of genus Sphaeroma, including species S. terebrans, S. retrolaeve, and S. serratum, was examined. The mitochondrial COl gene sequences of all specimens were sequenced and analysed. The interspecific Kimura 2-parameter distances were higher than intraspecific distances and no intraspecificinterspecific distance overlaps were observed. In addition, genetic distance and nucleotide diversity （TT） exhibited no differences within S. terebrans. Our results revealed that the mitochondrial COl gene can serve as a valid DNA barcode for the identification of S. terebrans. Furthermore, the number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod were found to be unreliable taxonomic characteristics for S. terebrans.