Effects of aspartame on hsp70,bcl-2 and bax expression in immune organs of Wistar albino rats

作     者：Arbind Kumar Choudhary Rathinasamy Sheela Devi 

作者机构：Department of PhysiologyDr.ALM.PG.Institute of Basic Medical SciencesUniversity of MadrasTaramani Campus 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2016年第30卷第5期

页      面：427-435页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the University of Madras for their financial support.[UGC No.D.1.(C)/TE/2012/1868 

主　　题：aspartame immune organs hsp70 bcl2 bax 

摘      要：Aspartame, a ＂first generation sweetener＂, is widely used in a variety of foods, beverages, and medicine. The FDA has determined the acceptable daily intake （ADI） value of aspartame to be 50 mg/kg, day, while the JECFA （Joint FAO/WHO Expert Committee on Food Additives） has set this value at 40 mg/kg of body weight/day. Safety issues have been raised about aspartame due to its metabolites, specifically toxicity from methanol and/or its systemic metabolites formaldehyde and formic acid. The immune system is now recognized as a target organ for many xenobiotics, such as drugs and chemicals, which are able to trigger unwanted apoptosis or to alter the regulation of apoptosis. Our previous studies has shown that oral administration of aspartame [40 mg/（kg, day）] or its metabolites for 90 days increased oxidative stress in immune organs of Wistar albino rats. In this present study, we aimed to clarify whether aspartame consumption over a longer period （90-days） has any effect on the expression ofhsp70, bcl- 2 and bax at both mRNA transcript and protein expression levels in immune organs. We observed that oral administration of aspartame for 90 days did not cause any apparent DNA fragmentation in immune organs of aspartame treated animals; however, there was a significant increase in hsp70 expression, apart from significant alteration in bcl-2 and bax at both mRNA transcript and protein expression level in the immune organs of aspartame treated animals compared to controls. Hence, the results indicated that hsp70 levels increased in response to oxidative injury induced by aspartame metabolites; however, these metabolites did not induce apoptosis in the immune organs. Furthermore, detailed analyses are needed to elucidate the precise molecular mechanisms involved in these changes.